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Sains Malaysiana 55(6)(2026): 1033-1044
http://doi.org/10.17576/jsm-2026-5506-08
Effects
of Cricket-Based Diet on Renal Oxidative Stress, Biochemical Profile, and
Histology in Protein-Malnourished Sprague-Dawley Weaned Rats
(Kesan Diet Berasaskan Cengkerik terhadap Tekanan Oksidatif Buah Pinggang, Profil Biokimia dan Histologi pada Tikus Sprague-Dawley yang Kekurangan Protein Selepas Cerai Susu)
SEE MENG LIM1, LE HAN WONG2,
XIN QIAN NG1 & IZATUS SHIMA TAIB2,*
1Centre
for Community Health Studies (ReaCH), Faculty of
Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia
2Centre
for Diagnostic, Therapeutic and Investigative Studies (CODTIS), Faculty of
Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz,50300 Kuala Lumpur, Malaysia
Diserahkan: 18 November 2025/Diterima: 3 Jun
2026
Abstract
Protein malnutrition impairs kidney
development during critical growth phases, increasing chronic kidney disease risk.
Edible crickets, rich in high-quality protein, offer a sustainable nutritional
intervention. This study evaluated a cricket-based diet (CBD) on renal
oxidative stress, biochemical profiles, and histology in protein-deficient
weaned Sprague-Dawley rats. Three diets were formulated: low protein diet (LPD;
6% protein), normal protein diet (NPD; 18% protein), and CBD (18% protein).
Three-week-old rats (n=8/group/sex) were randomly assigned to different groups:
male NPD (MN), male LPD (ML), male treatment (MT), male CBD (MC), and their
respective female counterparts (FN, FL, FT, FC). MN and FN received NPD, while
ML and FL were fed LPD for 10 weeks. MT and FT were given LPD for 3 weeks
followed by NPD for 7 weeks, and MC and FC received LPD for 3 weeks before CBD
for 7 weeks. No significant difference in body weight was observed between the
MN and MC groups, though FC was significantly lighter than FN. Notably, FC
exhibited significantly higher relative kidney weights compared to FN. Serum
urea levels in MC were elevated compared to ML, while no differences in serum
creatinine were found. ML demonstrated higher SOD activity than all other
groups. Histological analysis showed that MC and FC had improved glomerular
morphometry compared to ML and FL. Fibrosis persisted in both sexes. CBD
treatment partially ameliorated renal function and structure following
early-life protein malnutrition, with some sex-specific effects. Further
investigation is needed to optimise its efficacy and fully elucidate its
underlying mechanisms.
Keywords: Cricket-based diet;
kidney; oxidative stress; protein malnutrition; sex difference
Abstrak
Kekurangan protein menjejaskan perkembangan buah pinggang semasa fasa pertumbuhan kritikal, sekali gus meningkatkan risiko penyakit buah pinggang kronik. Cengkerik yang kaya dengan protein berkualiti tinggi menawarkan intervensi pemakanan yang mampan. Kajian ini menilai kesan diet berasaskan cengkerik (CBD) terhadap tekanan oksidatif buah pinggang, profil biokimia dan histologi dalam tikus Sprague-Dawley yang kekurangan protein selepas cerai susu. Tiga jenis diet telah disediakan: diet rendah protein
(LPD; 6% protein), diet protein normal (NPD; 18% protein) dan CBD (18%
protein). Tikus berumur tiga minggu (n=8/kumpulan/jantina) diagihkan secara rawak kepada kumpulan berbeza: jantan NPD (MN), jantan LPD (ML), jantan rawatan (MT), jantan CBD (MC) serta pasangan betinanya (FN, FL, FT, FC). MN dan FN menerima NPD, manakala ML dan FL diberi LPD selama 10 minggu. MT
dan FT diberi LPD selama 3 minggu diikuti NPD selama 7 minggu, manakala MC dan FC menerima LPD selama 3 minggu diikuti CBD selama 7 minggu. Tiada perbezaan signifikan dalam berat badan diperhatikan antara kumpulan MN dan MC, namun FC didapati lebih ringan secara signifikan berbanding FN. Menariknya, FC menunjukkan berat relatif buah pinggang yang lebih tinggi berbanding FN. Paras urea
serum dalam MC meningkat berbanding ML, manakala tiada perbezaan pada kreatinin serum diperhatikan. Aktiviti SOD lebih tinggi dalam ML berbanding semua kumpulan lain. Analisis histologi menunjukkan MC dan FC mempunyai morfometri glomerulus
yang lebih baik berbanding ML dan FL. Fibrosis masih kekal dalam kedua-dua jantina. Rawatan CBD sebahagiannya memperbaiki fungsi dan struktur buah pinggang selepas kekurangan protein awal hayat dengan beberapa kesan khusus jantina. Kajian lanjut diperlukan untuk mengoptimumkan keberkesanannya dan menjelaskan sepenuhnya mekanisme yang terlibat.
Kata kunci: Buah pinggang; diet berasaskan cengkerik; kekurangan protein; perbezaan jantina; tekanan oksidatif
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*Pengarang untuk surat-menyurat;
email: izatusshima@ukm.edu.my
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